Elu aha ọma na-ere ọkụ Proteinase K CAS 39450-01-6 Ogo nke ọma
Anyị na-eche ihe ahịa na-eche, ngwa ngwa nke ngwa ngwa na-eme ihe n'ime ọdịmma nke a ahịa ọnọdụ nke isi ụkpụrụ, na-enye ohere nke ukwuu mma elu àgwà, belata nhazi ụgwọ, ebubo ndị ọzọ ezi uche, merie ọhụrụ na gara aga ahịa nkwado na affirmation maka. Elu aha Hot ireProteinase K CAS 39450-01-6Nkwenye nke ọma, Dị ka ọkachamara ọkachamara n'ime mpaghara a, anyị agbaala mbọ idozi nsogbu ọ bụla nke nchebe oke okpomọkụ maka ndị ọrụ.
Anyị na-eche ihe ahịa na-eche, ngwa ngwa nke ngwa ngwa na-eme ihe n'ime ọdịmma nke a ahịa ọnọdụ nke isi ụkpụrụ, na-enye ohere nke ukwuu mma elu àgwà, belata nhazi ụgwọ, ebubo ndị ọzọ ezi uche, merie ọhụrụ na gara aga ahịa nkwado na affirmation maka.China Proteinase K na CAS 39450-01-6, E nwere elu na-emepụta & nhazi ngwá ọrụ na nkà na-arụ ọrụ iji hụ na ihe ndị dị elu mma.Anyị achọpụtala ihe magburu onwe ya tupu ire ere, ire ere, ọrụ ire ere iji hụ na ndị ahịa nwere ike ijide n'aka na ha ga-enye iwu.Ruo ugbu a, ngwọta anyị na-aga n'ihu ngwa ngwa ma na-ewu ewu na South America, East Asia, Middle East, Africa, wdg.
| Nke 1 mmiri mmiri | |
| Ọrụ kpọmkwem protein> 800U / ml, ntinye uche bụ 20mg / ml, ọ nweghị ihe fọdụrụ na Nickase. | |
| Nke 2 ntụ ntụ | |
| Ọdịdị | ntụ ntụ lyophilized ọcha |
| Electrophoretic ịdị ọcha | ≥95% |
| Enzyme ọrụ | ≥30U/mg |
| Nucleic acid fọdụrụnụ | A naghị ahụ anya |
| Ihe fọdụrụ deoxyribonuclease | A naghị ahụ anya |
| Ribonuclease fọdụrụnụ | A naghị ahụ anya |
| Ụgbọ ala Mba. | G1205-10ML |
| Nchekwa | Mgbe echekwara na -20 Celsius C, ngwaahịa ahụ na-ejigide ọrụ ma ọ dịkarịa ala 1 afọ, njem akpa ice. |
Proteinase Kbụ serine protease nke ezinụlọ subtilisin nwere ọrụ enzymatic na-arụ ọrụ nke ọma na nkọwapụta mkpụrụ osisi sara mbara, nke na-ahọrọ nke ọma decomposes ester bond na peptides n'akụkụ C-terminus nke amino acid hydrophobic, amino acid nwere sọlfọ nwere sọlfọ na njikọ amino acid aromatic, nke bụ. A na-ejikarị eme ka protein dị ala iji mepụta peptides dị mkpụmkpụ.Proteinase K nwere njirimara nke catalytic triad Asp39-His69-Ser224 pụrụ iche na serine proteases ma nwee saịtị abụọ Ca2 + na gburugburu ebe a na-arụ ọrụ iji mee ka nkwụsi ike ya dịkwuo elu ma nọgide na-arụ ọrụ enzymatic dị elu n'okpuru ọnọdụ dịgasị iche iche.
1. Ngwa nyocha mkpụrụ ndụ ihe nketa.
2. Ngwa RNA na DNA mmịpụta.
3. Wepụ ihe ndị na-abụghị protein na anụ ahụ ma na-emebi protein adịghị ọcha (dị ka nkwadebe nke ọgwụ DNA na heparin).
4. Nkwadebe nke Chromosomal DNA maka Pulse Electrophoresis.
5. Western blotting.
6. Mmepe na mmepụta nke enzymatic glycated albumin reagents na ubi nke in vitro diagnostics.
1. 30mg / karama 1g / karama ma ọ bụ 100g / karama
2. 1kgs / akpa foil
Proteinase K bụ serine protease kwụsiri ike nwere nkọwapụta mkpụrụ osisi sara mbara.Ọ na-eweda ọtụtụ protein na steeti ala ọbụna na ọnụnọ nke ncha.Ọ naghị arụ ọrụ ya site na ion ígwè, ndị na-emepụta chelating (dịka ọmụmaatụ, EDTA), sulfhydryl reagents, ma ọ bụ site na tripsin ma ọ bụ chymotrypsin inhibitors.Ọ kwụsiri ike karịa oke pH (4-12.5), yana ọrụ kachasị mma na pH 6.5-9.5.Enwere ike kpalite mmemme site na mgbakwunye nke ndị na-egbochi denaturing (SDS na Urea).Denaturation nke enzyme ngwa ngwa na-apụta na okpomọkụ karịa 70 Celsius.Autolysis nke enzyme na-emewanyewanye na pH alkaline.Agbanyeghị, Proteinase K adịghị arụ ọrụ kpamkpam site na autolysis.Ụfọdụ iberibe enzyme na-aga n'ihu na-ejigide ọrụ proteolytic ha zuru oke, ọbụlagodi mgbe nyochachara nke ukwuu.A na-ejikarị Proteinase K eme ihe na ngwa ihe ndị dị ndụ iji gbarie protein ndị a na-achọghị, dị ka nucleases na DNA ma ọ bụ nkwadebe RNA sitere na microorganisms, mkpụrụ ndụ omenala, na osisi.A na-ejikarị enzyme eme ihe na 50-200 ug/ml na nkwadebe nucleic acid na pH 7.5-8.0 na 37 ~ 55oC.Oge nnabata na-adị iche site na nkeji iri atọ ruo awa iri na asatọ.
Serine protease kwụsiri ike na nke ukwuu;eji maka protein na nucleic acid iche. A na-eji Proteinase K mee ka RNA na DNA dị ọcha site na anụ ahụ ma ọ bụ ahịrị sel.
Anyị na-eche ihe ahịa na-eche, ngwa ngwa nke ngwa ngwa na-eme ihe n'ime ọdịmma nke a ahịa ọnọdụ nke isi ụkpụrụ, na-enye ohere nke ukwuu mma elu àgwà, belata nhazi ụgwọ, ebubo ndị ọzọ ezi uche, merie ọhụrụ na gara aga ahịa nkwado na affirmation maka. Aha dị elu Hot ire Proteinase K CAS39450-01-6Nkwenye nke ọma, Dị ka ọkachamara ọkachamara n'ime mpaghara a, anyị agbaala mbọ idozi nsogbu ọ bụla nke nchebe oke okpomọkụ maka ndị ọrụ.
Aha dị eluChina Proteinase K na CAS 39450-01-6, E nwere elu na-emepụta & nhazi ngwá ọrụ na nkà na-arụ ọrụ iji hụ na ihe ndị dị elu mma.Anyị achọpụtala ihe magburu onwe ya tupu ire ere, ire ere, ọrụ ire ere iji hụ na ndị ahịa nwere ike ijide n'aka na ha ga-enye iwu.Ruo ugbu a, ngwọta anyị na-aga n'ihu ngwa ngwa ma na-ewu ewu na South America, East Asia, Middle East, Africa, wdg.
