ụlọ ọrụ mmepụta ihe dị ala Alkaline ProteinaseK CAS 39450-01-6

Anyị na-agba mbọ na-enye ndị asọmpi ọnụego, pụrụ iche ngwaahịa na ngwọta dị elu mma, kwa dị ka ngwa ngwa nnyefe maka factory ala price AlkalineProteinaseK CAS 39450-01-6, Anyị na-na-na na ezi obi chọrọ n'ihu na-arụkọ ọrụ na-eji ọkụ n'ebe nile n'ụwa dum. Anyị kwenyere na anyị ga-eju gị afọ. Anyị na-anabatakwa ndị na-azụ ahịa ka ha gaa na nzukọ anyị zụrụ ngwaahịa anyị.
Anyị na-ekwe nkwa na-enye ndị asọmpi ọnụego, pụrụ iche ngwaahịa na ngwọta elu àgwà, kwa dị ka ngwa ngwa nnyefe makaChina Alkaline ProteinaseK na 39450-01-6, N'ihi ezigbo mma na ọnụ ahịa ezi uche dị na ya, a na-ebupụ ngwaahịa anyị na mba na mpaghara 10 karịa. Anyị na-atụ anya nkwado ndị ahịa niile si n'ụlọ na mba ofesi. Ọzọkwa, afọ ojuju ndị ahịa bụ nchụso ebighi ebi anyị.

Proteinase Kbụ serine protease nke ezinụlọ subtilisin nwere ọrụ enzymatic na-arụ ọrụ nke ọma na nkọwapụta mkpụrụ osisi sara mbara, nke na-ahọrọ nke ọma decomposes ester bond na peptides n'akụkụ C-terminus nke amino acid hydrophobic, amino acid nwere sọlfọ nwere sọlfọ na njikọ amino acid aromatic, nke bụ. A na-ejikarị eme ka protein dị ala iji mepụta peptides dị mkpụmkpụ. Proteinase K nwere njirimara nke catalytic triad Asp39-His69-Ser224 pụrụ iche na serine proteases ma nwee saịtị abụọ Ca2 + na gburugburu ebe a na-arụ ọrụ iji mee ka nkwụsi ike ya dịkwuo elu ma nọgide na-arụ ọrụ enzymatic dị elu n'okpuru ọnọdụ dịgasị iche iche.

1. Ngwa nyocha mkpụrụ ndụ ihe nketa.
2. Ngwa RNA na DNA mmịpụta.
3. Wepụ ihe ndị na-abụghị protein na anụ ahụ ma na-emebi protein adịghị ọcha (dị ka nkwadebe nke ọgwụ DNA na heparin).
4. Nkwadebe nke Chromosomal DNA maka Pulse Electrophoresis.
5. Western blotting.
6. Mmepe na mmepụta nke enzymatic glycated albumin reagents na ubi nke in vitro diagnostics.

1. 30mg / karama 1g / karama ma ọ bụ 100g / karama
2. 1kgs / akpa foil

Proteinase K bụ serine protease kwụsiri ike nwere nkọwapụta mkpụrụ osisi sara mbara. Ọ na-eweda ọtụtụ protein na steeti ala ọbụna na ọnụnọ nke ncha. Ọ naghị arụ ọrụ ya site na ion ígwè, ndị na-emepụta chelating (dịka ọmụmaatụ, EDTA), sulfhydryl reagents, ma ọ bụ site na tripsin ma ọ bụ chymotrypsin inhibitors. Ọ kwụsiri ike karịa oke pH (4-12.5), yana ọrụ kachasị mma na pH 6.5-9.5. Enwere ike kpalite mmemme site na mgbakwunye nke ndị na-egbochi denaturing (SDS na Urea). Denaturation nke enzyme ngwa ngwa na-apụta na okpomọkụ karịa 70 Celsius. Autolysis nke enzyme na-emewanyewanye na pH alkaline. Agbanyeghị, Proteinase K adịghị arụ ọrụ kpamkpam site na autolysis. Ụfọdụ iberibe enzyme na-aga n'ihu na-ejigide ọrụ proteolytic ha zuru oke, ọbụlagodi mgbe nyochachara nke ukwuu. A na-ejikarị Proteinase K eme ihe na ngwa ihe ndị dị ndụ iji gbarie protein ndị a na-achọghị, dị ka nucleases na DNA ma ọ bụ nkwadebe RNA sitere na microorganisms, mkpụrụ ndụ omenala, na osisi. A na-ejikarị enzyme eme ihe na 50-200 ug/ml na nkwadebe nucleic acid na pH 7.5-8.0 na 37 ~ 55oC. Oge nnabata na-adị iche site na nkeji iri atọ ruo awa iri na asatọ.

Serine protease kwụsiri ike na nke ukwuu; eji maka protein na nucleic acid iche. A na-eji Proteinase K mee ka RNA na DNA dị ọcha site na anụ ahụ ma ọ bụ ahịrị sel.

Anyị na-agba mbọ ịnye ọnụego asọmpi, ngwaahịa pụrụ iche na ngwọta dị elu, dịkwa ka nnyefe ngwa ngwa maka ọnụ ahịa ụlọ ọrụ dị ala Alkaline ProteinaseKCAS39450-01-6, Anyị na-na-na na ezi obi chọrọ n'ihu na-arụkọ ọrụ na-eji ọkụ n'ebe nile n'ụwa dum. Anyị kwenyere na anyị ga-eju gị afọ. Anyị na-anabatakwa ndị na-azụ ahịa ka ha gaa na nzukọ anyị zụrụ ngwaahịa anyị.
factory ala priceChina Alkaline ProteinaseK na 39450-01-6, N'ihi ezigbo mma na ọnụ ahịa ezi uche dị na ya, a na-ebupụ ngwaahịa anyị na mba na mpaghara 10 karịa. Anyị na-atụ anya nkwado ndị ahịa niile si n'ụlọ na mba ofesi. Ọzọkwa, afọ ojuju ndị ahịa bụ nchụso ebighi ebi anyị.